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Amplification of negative strand splitzicon increases linearly with increasing doses of NSP. Negative strand was dosed at a 0.05 ug with increasing doses of NSP; (A) expressed as mean ± standard deviation RLU, (B) fold change compared to the control with no NSP and (C) linear fit of luciferase expression versus NSP dose. Dotted line at 1 represents no change over the no NSP control for the same condition.
