FIG 2 .

Reactivities of the 60 mouse antiserum-reactive chlamydial peptide antigens with C. trachomatis-positive (Ctr-Pos) and -negative (Ctr-Neg) human serum pools. The reactivity of each of the 60 Chlamydia species-specific peptide antigens (Fig. 1) was tested with the human serum pools. The C. trachomatis-positive pool consisted of sera from 125 women with C. trachomatis infection, and the C. trachomatis-negative pool consisted of sera from 17 women never diagnosed with C. trachomatis infection who were EB ELISA negative for anti-C. trachomatis antibodies. Polyclonal anti-human IgG HRP conjugate was used for detection of bound total IgG, and monoclonal antibody conjugates were used to detect bound long-lived IgG1 or short-lived IgG3 isotypes. Peptide reactivities are shown in Log₂ RLU signal bars. Different colors are used for the chlamydial species for convenient visualization.