FIG 3 .

Anti-C. trachomatis MIF titer-dependent IgG reaction intensity of the 60 chlamydial peptide antigens. C. trachomatis-positive sera from 108 women with known anti-C. trachomatis microimmunofluorescence (MIF) test results were combined by MIF titer into 4 subpools of 19 to 35 sera. Reactivities of the 4 subpools, indicated by color intensity, with peptides from all 11 chlamydial species (Fig. 1) are shown in Log₂ RLU IgG signal bars on the scale as described for Fig. 2. All 10 C. trachomatis peptides showed a signal above background, and the signals correlated highly significantly with MIF titers (R = 0.80; P < 10⁻⁶). Three C. pneumoniae peptides and six peptides from other Chlamydia species showed a signal above background that did not correlate with MIF titers (P = 0.78).