TABLE 3.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| Trans-T1 | Cloning strain | TransGen |
| BL21(DE3) | Expression strain | TransGen |
| Staphylococcus aureus | ||
| NCTC8325 WT | NCTC8325 WT strain | NARSAb |
| RN4220 | 8325-4; restriction modification deficient | NARSA |
| ΔmgrA | NCTC8325 mgrA mutant strain | This study |
| Δα/β | NCTC8325 psmα psmβ double mutant strain | This study |
| ΔmgrA/α/β | NCTC8325 mgrA psmα psmβ triple mutant strain | This study |
| Δagr | NCTC8325 agr mutant strain | This study |
| Δagr/mgrA | NCTC8325 agr mgrA double mutant strain | This study |
| N315 WT | N315 (agr-negative) WT strain | NARSA |
| N315ΔmgrA | N315 mgrA mutant strain | This study |
| NCTC8325-pli50 | NCTC8325 carrying plasmid pli50 | This study |
| ΔmgrA-pli50 | ΔmgrA carrying plasmid pli50 | This study |
| ΔmgrA-plimgrA | ΔmgrA carrying plasmid plimgrA | This study |
| Δα/β-pli50 | Δα/β carrying plasmid pli50 | This study |
| ΔmgrA/α/β-pli50 | ΔmgrA/α/β carrying plasmid pli50 | This study |
| NCTC8325 pGFP | NCTC8325 carrying plasmid pGFP | This study |
| Δα/β pGFP | Δα/β carrying plasmid pGFP | This study |
| ΔmgrA pGFP | ΔmgrA carrying plasmid pGFP | This study |
| ΔmgrA/α/β pGFP | ΔmgrA/α/β carrying plasmid pGFP | This study |
| Plasmids | ||
| pBTs | E. coli-S. aureus shuttle vector; temperature sensitive, Ampr, Cmr | 43 |
| pBTs-mgrA | pBTs plasmid containing mutant allele for mgrA deletion; Ampr, Cmr | This study |
| pBTs-psmα | pBTs plasmid containing mutant allele for psmα deletion; Ampr, Cmr | This study |
| pBTs-psmβ | pBTs plasmid containing mutant allele for psmβ deletion; Ampr, Cmr | This study |
| pli50 | E. coli-S. aureus shuttle vector; Ampr, Cmr | Addgene |
| plimgrA | Complete mgrA gene under control of native promoter in pli50; Ampr, Cmr | This study |
| pGFP | gfp expression with promoter of S10 ribosomal gene; Ampr, Cmr | 49 |
a
Ampr, ampicillin resistant; Cmr, chloramphenicol resistant.
b
NARSA, Network on Antimicrobial Resistance in Staphylococcus aureus.