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The dual T7-tac promoter plasmid for PPGK screening in E. coli TOP10 and protein expression in E. coli BL21(DE3). (A) The conceptual plasmid map of pET28a-Ptac-ppgk. The DNA sequences of Ptac, lac operator, and the RBS are shown as underlined, italic, and lowercase, respectively. (B) SDS-PAGE analysis of PPGK expression in E. coli TOP10 and E. coli BL21(DE3). M, protein marker; control, pET28a-Ptac; WT, pET28a-Ptac-ppgk; P, purified PPGK. PPGK is indicated with an arrow.
