TABLE 1.

Kinetic parameters of V. boronicumulans CGMCC 4969 nitrile-converting enzymes^a

Enzyme	Vmax (U · mg−1)	Km (mmol · liter−1)
NHase	30.82 ± 1.64	2.87 ± 0.31
IamA	13.09 ± 1.05	0.52 ± 0.08
NitA	23.67 ± 2.51	0.77 ± 0.12

^aThe kinetic parameters of the nitrile-converting enzymes were estimated over a range of substrate concentrations (0.1 to 6 mmol · liter⁻¹) at 37°C in 50 mmol · liter⁻¹ phosphate buffer at pH 7.5. Data shown are mean values ± standard deviations from three replicates.