Figure 2.

CT288 and CCDC146 interact after ectopic expression in mammalian cells. Plasmids encoding the indicated proteins were used to transfect HEK293T cells for 24 h. The cells were then lysed and EGFP proteins were pulled-down using GFP-Trap (ChromoTek). Proteins in the input lysate and in the pull-down output fractions were analyzed by immunoblotting using anti-HA and anti-GFP antibodies. (A) EGFP-CCDC146_692−955 and EGFP-CCDC146_FL, but not EGFP alone, co-immunoprecipitate CT288_ΔNΔTMD-HA. (B) EGFP-CT288_ΔNΔTMD, but not EGFP alone, co-immunoprecipitates CCDC146_FL-HA and CCDC146_692−955-HA. The arrows indicate the position in the blots of the relevant proteins (CT288_ΔNΔTMD-HA, predicted molecular mass of 50 kDa; EGFP, 28 kDa; EGFP-CCDC146_692−955, 60 kDa; EGFP-CCDC146_FL, 140 kDa; EGFP-CT288_ΔNΔTMD, 80 kDa; CCDC146_FL-HA, 110 kDa; CCDC146_692−955-HA, 30 kDa). The whole blots are shown in Figures S2, S3.