Figure 3.

CT288-2HA produced by C. trachomatis during infection interacts with EGFP-CCDC146_692−955. Plasmids encoding EGFP or EGFP-CCDC146_692−955 were used to transfect HEK293T cells that were also infected for 24 h with C. trachomatis harboring pCT288-2HA. The cells were then lysed and EGFP proteins were pulled-down using GFP-Trap (ChromoTek). Proteins in the input lysate and in the pull-down output fractions were analyzed by immunoblotting using anti-HA and anti-GFP antibodies. The arrows indicate the position in the blots of the relevant proteins (CT288-2HA, predicted molecular mass of 66 kDa; EGFP, 28 kDa; EGFP-CCDC146_692−955, 60 kDa). The whole blots are shown in Figure S5.