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. 2018 Jul 20;9(7):366. doi: 10.3390/genes9070366

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Integrase induction drives excision of G08 and G62 mini-islands in E. coli and A. baumannii. Gel images of circularized G08 (from AB0057) (A) and G62 (from ATCC 17978) (B) mini-islands respectively, with (+) and without (−) IPTG induction. Mini-GI excision was tested by amplification of circular intermediates using outwards facing primer sets yielding 567 bp product for G08 (A) and a 489 bp product for G62 excision (B). Different time points (in hours) after IPTG induction in early exponential phase are shown (legend above gel). Integrase dependent excision of G08 and G62 mini-islands in A. baumannii is shown in panel C. Gel images of circularized G08 and G62 mini-islands 8 h after IPTG induction with the same primers as in E. coli (panel A and B). The non-induced samples in lane 3 for G8 and in lane 5 for G62 in lanes 4 and 5 of panel C. The marker is Gene Ruler 1 kb and the size of some bands in bp is given on the left of each gel.