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. 2018 Jul 6;10(7):359. doi: 10.3390/v10070359

Figure 6.

Figure 6

FUS is predominately nuclear in KSHV-infected cells. (A) TREx-BCBL1-RTA cells were fractionated at latency and 48 h post-reactivation. WCE: Whole Cell Extract. Cyto: Cytoplasm. Hsp90 is a cytoplasmic control, H3 is a nuclear control. Quantification is to the right of the western blot. (B) Fractionation of iSLK.219 cells. (C) IF, at 60× magnification, of iSLK.BAC16 cells at latency and 72 h post-reactivation. DAPI is a nuclear marker. (D) Chromatin fractionation of iSLK.219 cells at latency and 48 h post-reactivation. WCE: whole cell extract. N.P.: nucleoplasm. Hsp90 is a cytoplasmic control, H3 a nuclear control. Quantification is to the right of the Western blot.