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. 2018 Jul 21;7(7):81. doi: 10.3390/cells7070081

Figure 1.

Figure 1

(A) Relative AQP1 transcript expression in untreated HT-29, HCT116, SW480, and SW620 colon cancer cell lines. Transcript expression was calculated using the ΔΔCt relative quantification method, normalising to PMM1 reference gene. Results are the mean ± SD of biological triplicates, relative to the expression in HT-29. (B) Representative western immunoblot for AQP1 protein expression in untreated HT-29, HCT116, SW480, and SW620 colon cancer cell lines. (C) Relative AQP1 protein expression in untreated HT-29, HCT116, SW480, and SW620 colon cancer cell lines. AQP1 expression was normalised to beta-actin loading control and relative to the expression in HT-29.