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. 2018 Jul 20;10(7):301. doi: 10.3390/toxins10070301

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The role of Rum1 in aflatoxin biosynthesis. (A) AFB₁ production of WT, ∆rum1, and ∆rum1-C strains was detected by Thin-Layer Chromatography (TLC). All strains were cultivated in liquid YES medium for 6 days at 29 °C in the dark (5 μL of each sample was allotted for the TLC analysis. Three lanes of the same strain represent biological repeats, respectively). (B) The quantity of AFB₁ produced by related A. flavus strains was quantitated according to (A), (C) Transcriptional levels of aflatoxin biosynthesis related gene aflR, aflS, aflC, and aflO in the strains mentioned above. *** represents a significant difference (p < 0.001).