Figure 6.

The role of Rum1 in A. flavus colonization. (A) Photographs presented the maize and peanut kernels colonized by WT, ∆rum1, and ∆rum1-C strains. (B) Conidia production of all the strains on seeds (The conidia of each plate were suspended with 5 mL of spore eluate and the conidia number of A. flavus strains in 1 mL spore eluate was calculated with hemocytometer). (C) The production of AFB₁ from colonized kernels was detected by TLC (5 μL from each sample was loaded for the TLC analysis. Three lanes of the same strain represent three biological repeats, respectively). (D) Relative AFB₁ production in (C) was quantitated. (E) Amylase activity was analyzed with iodine solution and 1% starch (Red arrows point to the degradation zones). (F) The relative degradation rate of starch of WT, ∆rum1, and ∆rum1-C strains, according to the results of (E). *** represents significant difference (p < 0.001).