. 2018 Jul 20;10(7):301. doi: 10.3390/toxins10070301

Table 2.

Primers used for the strain construction in this study.

Primer Name	Sequence (5′-3′)	Fragment
rum1-p1	GGCACGAGCTATTAGTGATATTAGTCGAGTCCGA	5′UTR of rum1
rum1-p2	CAAGTGAGCCGACCGATTGAGGGAAGTAGT	5′UTR of rum1
rum1-p3	TCCCTATCAACAAATTGGCGCTTCATGGGTTC	3′UTR of rum1
rum1-p4	TGGATTCCTTCGGGGGCTAGTTTGCATC	3′UTR of rum1
rum1-p5	ACTACTTCCCTCAATCGGTCGGCTCACTT	A. fumigatus pyrG
rum1-p5	GGCCTCAAACAATGCTCTTCACCC
rum1-p6	GAACCCATGAAGCGCCAATTTGTTGATA
rum1-p6	GGGAGTCTGAGAGGAGGCACTGATGC
rum1-p7	GACCTGTGAAGATGCTTGGTAGAGCTATTTCAG	Nesting primers
rum1-p8	TATCTCATTGGACTGGACCCTGAGCGGGA	Nesting primers
rum1-p9	CAACTCGACTGGCGGACAGCCT	A fragment from rum1
rum1-p10	TCATTTGCCGGAGAATATGTTCCAGTCCTTC	A fragment from rum1
P801	CAGGAGTTCTCGGGTTGTCG	A. fumigatus pyrG
P1020	CAGAGTATGCGGCAAGTCA	A. fumigatus pyrG
rum-p3 + pyrg-F	CTTCATCGCGAGATAACACCCCCGATGG	5′UTR of Δrum1-C
rum-p3 + pyrg-R	GGGTGAAGAGCATTGTTTGAGGCCCCATG	5′UTR of Δrum1-C
pyrg-F	GCCTCAAACAATGCTCTTCACCC	A. fumigatus pyrG
pyrg-R	GTCTGAGAGGAGGCACTGATGC	A. fumigatus pyrG
rum-p4 + pyrg-F	GCATCAGTGCCTCCTCTCAGACAGATTCTT	3′UTR of Δrum1-C
rum-p4 + pyrg-F	GCCTTGCGCATTCATGACAAC	3′UTR of Δrum1-C