Skip to main content
. 2018 Jul 17;9(7):357. doi: 10.3390/genes9070357

Figure 3.

Figure 3

Effect of GRP78 overexpression in the VMH of vHFD rats on gonadal white adipose tissue (gWAT) browning. (A) Representative immunohistochemistry with anti-UCP1 antibody showing UCP1 staining (left panels; original magnification 20×; scale bar 100 µm), UCP1 stained area (right upper panels; n = 8–10 animals per group), adipocyte area (right lower panels; n = 8–10 animals per group) and (B,C) relative messenger RNA (mRNA) levels of thermogenic markers (n = 8–10 animals per group) in the gWAT of the STD or vHFD rats stereotaxically treated with GFP or GRP78 adenoviruses into the VMH. Statistical significance was determined by t-Student test or ANOVA. Error bars represent the SEM. * p < 0.05 and *** p < 0.001 vs. (STD or vHFD) Ad GFP; # p < 0.05 and ## p < 0.01 vs. vHFD Ad GFP (panel A).