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Desmosome forces are increased in epithelial acini. (A) MDCK cells expressing the DSG-2 tension sensor were either seeded in Matrigel for seven to ten days to form acini or on top of thin Matrigel to form monolayers. Scale bar is 20 microns. (B) Quantification of FRET differences showed acini have an increased DSG-2 force compared to monolayers (representative experiment of three experiments with similar results, ANOVA with Newman-Keuls post-hoc test, * p < 0.01).
