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. 2018 Jul 20;10(7):381. doi: 10.3390/v10070381

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Salivary gland extract from Hyalomma ticks inhibits migration of human monocyte-derived dendritic cells (moDC). moDC migration was studied in a trans-well migration assay. When moDC where exposed to salivary gland extract (SGE) fewer cells migrated to the other side of the trans-well (mock = RPMI only) or with chemoattractant (CCL19). Number of migrated cells was assessed by lysing cells and analyzing DNA content by intercalating dye. Fluorescent was measured as mean fluorescent intensity (MFI). Significance between mock and indicated treatment groups at p < 0.05 and p < 0.01 is designated with one or two asterisk (*) symbols, respectively.