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Fluorescence quenching of human serum albumin (HSA) by ochratoxins. Emission spectra of HSA (2 μM) in the presence of increasing 2′R-OTA (A: 0.0, 0.1, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 2.0, 3.0, and 5.0 μM) and OTA (B: 0, 0.1, 0.25, 0.5, 0.75, 1.0, 1.25, 1.5, 2.0, 3.0, and 5.0 μM) concentrations in PBS (pH 7.4; λex = 295 nm). Ochratoxin-induced decrease of the fluorescence emission signal of HSA at 340 nm (C) (n = 3). RLU: Relative Light Unit.
