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Mean residue ellipticity and α-helix contents of 0.7 µM HSA in presence of 14 µM OTA and 14 µM 2′R-OTA in PBS. α-helix percentage calculated with Equation (15) * and with K2D3 software **.
| HSA + Ochratoxin (Ratio) |
θMRE (×102) (deg × cm2 × dmol−1) |
α-Helix * (%) | α-Helix ** (%) | Rel. Differences to HSA | ||
|---|---|---|---|---|---|---|
| 208 nm | 222 nm | 208 nm | 222 nm | |||
| HSA | −247 | −228 | 71.4 | 65.0 | 66.9 | - |
| HSA + 2′R-OTA (1:20) | −220 | −210 | 61.8 | 58.5 | 65.3 | 2–13% |
| HSA + OTA (1:20) | −206 | −209 | 57.3 | 58.3 | 61.5 | 8–20% |
