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. 2018 Jul 23;8(7):127. doi: 10.3390/ani8070127

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression levels of MyoD, MyoG, Mef2c and MSTN in myoblasts transfected with GV287-TCONS_00102859. To investigate the influence of the overexpression of TCONS_00102859 on myoblasts, GV287-TCONS_00102859, for which stable expression can be achieved, was used. Cells transfected with GV287-N empty vector were set as a control. We detected four genes related to muscle development. Among these, the expression levels of MyoD, MyoG and Mef2c, which had a positive impact on myogenesis, were significantly higher than those of the control (p < 0.01). Meanwhile, the expression level of MSTN, which can inhibit myogenesis, was significantly lower than that of the control (p < 0.01). ‘*’ represents significant difference (p < 0.05) and ‘**’ represents extremely significant difference (p < 0.01).