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. 2018 Jul 16;9(7):353. doi: 10.3390/genes9070353

Figure 5.

Figure 5

Chordin (CHRD) genes encoding proteins with domain architectures typical of vertebrate chordins are present in the genomes of B. belcheri, B. floridae, and A. lucayanum. (A) The characteristic domain architecture of vertebrate chordins is conserved in B. floridae (Q0Q581), but the structure of the B. belcheri ortholog (Bb_056190F) is markedly different in that it lacks some of the domains and has a long C-terminal extension containing a polycystic kidney disease (PKD)_channel domain. The transcriptome of A. lucayanum contains full-length transcripts of the CHRD gene (GETC01124733.1). The transcriptome of B. lanceolatum had several transcripts of the CHRD gene and one of them matching the C-terminal part of the protein (JT869941.1) supports the conclusion that the protein does not have a PKD_channel domain; (B) Re-annotation of the B. belcheri CHRD gene with the MisPred/FixPred protocol has shown that these deviations are due to errors in gene prediction, permitting the correction of this sequence (CHRD_BRABE). The figure illustrates the Pfam domain architectures of the proteins and position of protein fragments of chordins. Color code for Pfam-A domains: von Willebrand factor type C domain (VWC)—green; chordin (CHRD)—red; PKD_channel—blue.