Figure 6.

DCT response to TAL dysfunction. A) Transmission electron micrographs of a DCT in 20-wk-old Gla^tmTg(CAG-A4GALT) and WT mice. Arrows indicate elongated mitochondria and an extensive basolateral membrane (infolding). Scale bars, 1 μm. B) Real-time RT-PCR analysis of Slc12a3 (NCC) in the same mice as in Fig. 4A. C) Western blot analysis of NCC expression in the same mice as in Fig. 4B. D) Representative images of NCC immunoreactivity in Gla^tmTg(CAG-A4GALT) and WT mice (n = 3/group). Scale bars, 1 mm (left), 50 μm (middle), and 10 μm (right). E) Representative images of Na⁺-K⁺-ATPase immunoreactivity in a DCT of Gla^tmTg(CAG-A4GALT) and WT mice (n = 3/group). Scale bars, 10 μm. In box-and-whisker plots (B, C), center lines represent the median, limits represent quartiles, whiskers represent the 10th and 90th percentiles, and red lines represent the mean. Differences between groups were evaluated with the Student’s t test; data are shown as t (integral degree of freedom) = t, P. For Welch’s t test, data are shown as t (mixed decimal degree of freedom) = t , P. For the Wilcoxon rank-sum test, data are shown with a P value only.