Figure 1.

Developing monoclonal antibody against topoI-pS10: (A) Schematics representing various steps to develop mouse monoclonal antibody specific to phosphorylated topoI-S10. (B) FFPE slides of colon cancer cell lines were immunostained with hybridoma cell culture media to identify the antigen-specific clones. Scale bar = 100 µm. Abbreviations: topoI-pS10, topoisomerase I phosphorylates at serine 10; MAb, monoclonal antibody; RIMMS, Repetitive Immunizations Multiple Sites; FFPE, formalin-fixed paraffin embedded.