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. 2018 Mar 27;66(8):549–561. doi: 10.1369/0022155418766503

Figure 2.

Figure 2.

AR optimization. (A, B, and C): Serial sections of breast cancer FFPE slides were subjected to AR at different temperatures for immunostaining with anti-topoI-pS10. (D) CRC FFPE slides were also subjected to immunostaining with anti-topoI-pS10 without heat-induced AR. (E) The same tissue was immunostained with anti-topoI-pS10 after heat-induced AR at 100C. The red box is an enlarged image of the positive area. Scale bar = 100 µm. Abbreviations: AR, antigen retrieval; FFPE, formalin-fixed paraffin embedded; topoI-pS10, topoisomerase I phosphorylates at serine 10; CRC, colon cancer.