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Determination of the sensitivity of FcaGHV1 gB PCR. Second-round FcaGHV1 PCR products from PBMCs of a domestic cat and the spleen and PBMCs of a Tsushima leopard cat. bp = base pairs; (-) = FcaGHV1 negative PBMC control; 1 = 1 µg DNA used in first round; 2 = 0.1 µg DNA; 3 = 1 × 10−2 µg DNA; 4 = 1 × 10−3 µg DNA; 5 = 1 × 10−4 µg DNA; 6 = 1 × 10−5 µg DNA.
