Table 1.

List of primers used to generate expression vectors for native STh and STp, and mutant STh peptides. ST encoding sequences are shown in lower case, and vector sequences are in upper case. Stop codons are underlined.

Primer	Nucleotide Sequence
STh-F	tgtaatcctgcttgtaccgggtgctattaaGGCGCCATGGGCAAAGTG
STp-F	taatcctgcttgtgctggttgctattaaGGCGCCATGGGCAAAGTG
STh-N12I-F	tgtatccctgcttgtaccgggtgctattaaGGCGCCATGGGCAAAGTG
STh-N12S-F	tgtagccctgcttgtaccgggtgctattaaGGCGCCATGGGCAAAGTG
STh-A14S-F	tgtaatcctagctgtaccgggtgctattaaGGCGCCATGGGCAAAGTG
STh-A14T(a)-F	tgtaatcctacttgtaccgggtgctattaaGGCGCCATGGGCAAAGTG
STh-A14T(b)-F	tgtaatcctacatgtaccgggtgctattaaGGCGCCATGGGCAAAGTG
DsbC-TAA-F	taaGCCATGGGCAAAGTGAGC
STh-R	acacaattcacagcagtaattgctactattCTGAAAATAAAGATTCTCGCTACCCG
STp-R	caacacaattcacagcagtagaaggtattCTGAAAATAAAGATTCTCGCTACCCG
STh-L9A-R	acatgcttcacagcagtaattgctactattCTGAAAATAAAGATTCTCGCTACCCG
STh-L9F-R	acaaaattcacagcagtaattgctactattCTGAAAATAAAGATTCTCGCTACCCG
STh-L9N-R	acagttttcacagcagtaattgctactattCTGAAAATAAAGATTCTCGCTACCCG
STh-L9S-R	acaactttcacagcagtaattgctactattCTGAAAATAAAGATTCTCGCTACCCG
STh-N12S-R	acacagttcacagcagtaattgctactattCTGAAAATAAAGATTCTCGCTACCCG
DsbC-TAA-R	GCCCTGAAAATAAAGATTCTCGC