Table 2.
Expression vectors for native STh and STp, and STh mutants.
| Plasmid | Forward Primer | Reverse Primer | ST Variant |
|---|---|---|---|
| pET-DsbC-STh | STh-F | STh-R | STh |
| pET-DsbC-STp | STp-F | STp-R | STp |
| pET-DsbC-STh-L9A | STh-F | STh-L9A-R | STh-L9A |
| pET-DsbC-STh-L9F | STh-F | STh-L9F-R | STh-L9F |
| pET-DsbC-STh-L9N | STh-F | STh-L9N-R | STh-L9N |
| pET-DsbC-STh-L9S | STh-F | STh-L9S-R | STh-L9S |
| pET-DsbC-STh-N12S | STh-N12S-F | STh-N12S-R | STh-N12S |
| pET-DsbC-STh-N12I | STh-N12I-F | STh-R | STh-N12I |
| pET-DsbC-STh-A14S | STh-A14S-F | STh-R | STh-A14S |
| pET-DsbC-STh-A14T | STh-A14T(a)-F | STh-R | STh-A14T |
| pET-DsbC-STh-L9S/A14T | STh-A14T(b)-F | STh-L9S-R | STh-L9S/A14T |