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. 2018 Jul 19;10(7):237. doi: 10.3390/cancers10070237

Figure 5.

Figure 5

Transfer of EBV miRNAs to the recipient cells cocultured with Mutu cells. Mutu−, Mutu I, or Mutu III cells were cocultured with AGS cells through trans-well inserts for 72 h. RNAs were isolated from AGS cells and subjected to stem-loop RT-PCR to analyze copy numbers of miR-BHRF1-1 (A); miR-BART7 (B); miR-BART10 (C); and miR-BART15 (D); To determine the role of exosomes in transfer of miR-BART10 to recipient cells, Mutu III cells were cocultured with AGS cells in the presence or absence of GW4869 (E). The experiment was performed three times independently and the average and its SD are shown in each condition. The average copy number of miRNA is shown. ** p < 0.01 vs. respective control (Student’s t-test).