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. 2018 Jul 11;10(7):365. doi: 10.3390/v10070365

Figure 6.

Figure 6

AMS but not MG132 effectively blocked baculovirus-expressed flavivirus E proteins induced syncytia. (A) AMS cytotoxicity was assessed by the viability of Sf9 cells treated with AMS. Pre-seeded Sf9 cells were treated with increasing concentrations of AMS for 24 h, and then subjected to cell viability analysis using CCK-8. (B) MG132 cytotoxicity was assessed by the viability of Sf9 treated with MG132. Pre-seeded Sf9 cells were treated with increasing concentrations of MG132 for 24 h, and then subjected to cell viability analysis using CCK-8. (C) The effect of AMS on the infectivity of baculovirus and expression of heterologous protein were evaluated using control baculovirus vAc-hsp70-egfp which expressed enhanced green fluorescent protein (egfp). (D) The effect of MG132 on the infectivity of baculovirus and expression of heterologous protein were evaluated using control baculovirus vAc-hsp70-egfp which expressed egfp. Bars, 100 μm. (E) AMS effectively blocked baculovirus-expressed flavivirus E proteins induced syncytia. (F) MG132 had no effect on baculovirus-expressed flavivirus E proteins induced syncytia. The number of syncytia within a field of view was counted for statistical analysis. The means and SD correspond to data collected from five random fields of view.