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F4N3Bzox-AP, 11-aziAP and alphaxalone modulate agonist binding to human α1β3γ2 GABAA receptors. The specific binding of 2 nM [3H]muscimol to membrane suspensions of GABAA receptors heterogeneously expressed in HEK293 cells was evaluated as a function of steroid concentration. Each data point was determined in triplicate and is plotted as the mean ± standard deviation.
