Skip to main content
. Author manuscript; available in PMC: 2018 Aug 18.
Published in final edited form as: Eur J Med Chem. 2017 Apr 21;136:334–347. doi: 10.1016/j.ejmech.2017.04.043

Figure 5.

Figure 5

11-F4B3Bzox-AP and 11-AziAP potentiate [3H]azietomidate and [3H]R-mTFD-MPAB photolabeling of α1β3γ2 GABAAR similar to alphaxalone or GABA. Affinity-purified α1β3γ2 GABAA receptors (~ 2 pmol of muscimol sites in 35 μL/lane) were equilibrated with 3 μM [3H]azietomidate or 1 μM [3H]R-mTFD-MPAB in the absence or presence of various drugs. After irradiation, the samples (in duplicate except for etomidate and R-mTFD-MPAB in B) were separated by SDS-PAGE and the stained GABAAR subunits were excised for 3H quantification by liquid scintillation counting. Means and ranges for the duplicate samples are plotted.