Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — SM (super module) Destination binary vectors and sgRNA shuffle-in vectors. a Schematic representation of SM Destination binary vectors. Plant codon optimized Cas9 (pcoCas9) is driven by the promoter of UBQ10 (proUBQ10). Transcriptional termination sequences from rbcS. Blue-white selection strategy with LacZ cassette. Seed coat expressed red fluorescence from At2S3:mcherry cassette as transgenic plant selection marker. Not drawn to scale. b Schematic representation of sgRNA shuffle-in vectors. sgRNA including 20-bp target sequences and shared sgRNA sequences transcribed by A. thaliana U6 promoter. c Overhang sequences used for Golden gate cloning in generating the final binary vectors. The numbers listed in left column are indicated in a and b