Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 2;11:44. doi: 10.1186/s13072-018-0213-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — EZH2-knockdown hDPCs show decreased proliferation, but no transcriptionally significant changes are observed in DNA replication-related genes. a Quantitative PCR and western blot show EZH2 knockdown with shRNA in hDPCs (72 h after transduction). b Colony formation assay by crystal violet staining demonstrates the viability of EZH2 knockdown hDPCs. c Growth curves measured with CCK-8. d Flow cytometry analysis shows the cell cycle distribution of EZH2-knockdown hDPCs. e Cell cycle RNA-sequencing assay shows the transcripts with significantly altered expression in EZH2-knockdown hDPCs. f Quantitative PCR verifies the expression levels of genes identified in the RNA-Seq data. (ns not significant, *P < 0.05, **P < 0.01, ***P < 0.001)