Fig. 5.

Lipid accumulation in the livers of CHOL-fed rabbits. a Liver triglycerides were quantified by MR spectroscopy (MRS) with selection of a voxel in images of the liver in vivo. The proton spectrum (inset in a) shows a major peak for water and a minor peak for the methylene groups of the fatty acyl chains in triglycerides. b MRS measurements of triglycerides in the livers of rabbits at 2 and 3 months were significantly higher in 1% CHOL-fed rabbits with endothelial injury versus 1% CHOL-fed rabbits with no injury (P < 0.05). Note that both groups were fed a normal chow diet for the last month (between 2 and 3 months). Representative MRS derived spectrums of liver water and fat in 1% CHOL-fed with no injury (c; 2.4% fat) and 1% CHOL + injury (d; 15.4% fat) at 3 months