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. 2018 Jul 26;9:1688. doi: 10.3389/fmicb.2018.01688

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Copyright © 2018 Ongey, Giessmann, Fons, Rappsilber, Adrian and Neubauer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro trypsin activation of His6-SUMO-preRumA and bioassay. (A) SDS-PAGE analysis of IMAC-purified modified His6-SUMO-preRumA^∗ (lane 1), trypsin-digested modified His6-SUMO-preRumA^∗ (lane 2), IMAC-purified non-modified His6-SUMO-preRumA^∗ (lane 3) and trypsin-digested non-modified His6-SUMO-preRumA^∗ (lane 4). (B) Bioassay analysis of the trypsin digested products of modified and non-modified His6-SUMO-preRumA^∗ against B. subtilis ATCC 6633 grown on a culture plate. (C) Mass spectrum showing prominent ion peak corresponding to active RumA purified from the trypsin digest of cyclized preRumA^∗ and bioactivity of the resulting product.