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Growth and product optimization. Growth curves of strain WLEOrM1 (A) and strain WLEOrM’ (B) cultures induced with varying IPTG concentrations in TB. (C) Expression of His6-RumM in pCTUT7 (strain WLEOrM’) versus pCUT7 (strain WLEOrM1). The concentrations of IPTG are indicated in the plots and at the top of each lane on the gel. The optimal IPTG concentration range is 50–100 μM.
