Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jul 26;9:1688. doi: 10.3389/fmicb.2018.01688

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Ongey, Giessmann, Fons, Rappsilber, Adrian and Neubauer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

TEV cleavage analyses and Native PAGE. (A) SDS-PAGE analysis of gel filtration-purified His6-RumM/His6-GFP-TEV-preRumA^∗ complex (lane 1), free His6-GFP-TEV-preRumA^∗ (lane 2), TEV digest of complex (lane 3) and TEV digest of free His6-GFP-TEV-preRumA^∗ (lane 4). (B) Native PAGE analysis of purified His6-GFP-TEV-preRumA from WLEOgrA^∗ strain (lane N1) and the His6-RumM/His6-GFP-TEV-preRumA complex sample (lane N2). Lane N2 shows multiple bands above the expected molecular weight of His6-RumM corresponding to 1 or 2 molecules of His6-GFP-TEV-preRumA^∗ complexed to one molecule His6-RumM.