. 2018 Jul 26;9:1688. doi: 10.3389/fmicb.2018.01688

Table 1.

Description of microbial strains used in this study.

Name of strain	Description	Purpose	Source
E. coli W3110	Wild type-like E. coli: F⁻λ⁻ rp/z-I INV(rrnD, rrnE)	Expression host	Stock center, Yale
Ruminococcus gnavus El	Wild type	Isolation of genomic DNA	CNRS, Marseille
B.subtilis ATCC 6633	–	Biological activity assay	DSM 347
E. coli Top 10	F^-mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80lacZΔM15 ΔlacX74 recAl araD139 Δ(ara leu) 7697 galU galK rpsL (StrR) endAl nupG	Plasmid storage	Invitrogen
WLEOv4	Cm^R, W3110 carrying pLEOrA	Expression of His6-preRumA	This work
WLEOrM’	Cm^R, W3110 carrying pLEOrM’	Expression of His6-RumM	This work
WLEOrMi	Cm^R, W3110 carrying pLEOrM1	Expression of His6-RumM	This work
WLEOM/M	Kan^R, Cm^R, W3110 carrying pLEOrA and pLEOrM	Coexpression of His6-preRumA and His6-RumM on separate plasmid	This work
WLEOgrv4	Cm^R, W3110 carrying pLEOgrA	Expression of His6-GFP-TEV-preRumA	This work
WLEOgrv4/M	Kan^R, Cm^R, W3110 carrying pLEOgrA and pLEOM	Coexpression of His6-GFP-TEV-preRumA and His6-RumM on separate plasmid	This work
WLEOgr.4^∗	Cm^R, W3110 carrying pLEOgrA^∗	Expression of His6-GFP-TEV-preRumA^∗	This work
WLEOgrA^∗Ml	Cm^R, W3110 carrying pLEOgrA^∗M1	Coexpression of His6-GFP-TEV-preRumA^∗ and His6-RumM on the same plasmid	This work
WLEOsrA^∗	Cm^R, W3110 carrying pLEOsrA^∗	Expression of His6-SUMO-preRumA^∗	This work
WLEOsrA^∗M	Cm^R, W3110 carrying pLEOsrA^∗M	Coexpression of His6-SUMO-preRumA^∗ and His6-RumM on the same plasmid	This work