Table 2.

Purification yields of total protein per liter.

Purification step	His6-GFP-TEV-preRumA (mg)	His6-GFP-TEV-preRumA∗ (mg)	¤His6-GFP-TEV-preRumA∗ (mg)	mpreRumA (mg)	∗(mg)	¤preRumA∗ (mg)
IMAC	84.53	97.61	97.61	–	–	–
Size exclusion	65.32	39.01	79.40	–	–	–
TEV-digested IMAC FT	–	–	–	3.23	4.45	6.14
TEV-digested butanol extract	–	–	–	1.34	2.50	4.75

The symbol ¤, pooled fractions from peaks A^′ and B^′ of Figure 4D. This specifically applies to His6-GFP-TEV-preRumA^∗ which appeared in two separate chromatographic peaks during purification. FT, flow-through. Concentrations were determined using the Bradford and the NanoDrop techniques.