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. 2018 Jun 13;8(14):3824–3840. doi: 10.7150/thno.25308

Figure 1.

Figure 1

FTY720 inhibited the growth and induces apoptosis in pancreatic cancer cell lines. (A) Human and mouse pancreatic cancer cells were treated with indicating concentrations of FTY720 (for 24 and 48 h) and cell survival was analyzed using MTT assay. (B) MIA PaCa-2 cells were treated with FTYT720 (10µM and 15µM) and the rate of apoptosis was quantified after 24h using annexin V-FITC staining. Scatter plot from FACS (upper panel) and quantification of live and apoptotic population (lower panel).(C) The effect of FTY720 on cell cycle was quantified using propidium iodide staining after treating MIA PaCa-2 cells with 10µM of FTY720. (D) MIA PaCa-2 cells were treated with FTY720 alone and the increased ROS positive population was quantified using DCFDA staining. (E) The anti-migratory capability of FTY720 was checked using wound healing assay. Briefly, a scratch was made when the cells reach 90% confluence and treated with an increased dose of FTY720 and the closure of wound was monitored at 12h and 24 h of time intervals. Representative images (left panel) and quantification (right panel). Data is presented as mean ± S.D and representative data from at least 3 independents experiments is shown, Statistical significance was calculated using t-test * p≤0.001.