Figure 5.

FTY720 sensitizes tumors towards gemcitabine through altered desmoplasia and reduced Shh signaling. (A) Representative images of tissue sections of α-SMA (top panel), quantification of positive cells (right panel), Sirius red (central panel), quantification of fibrotic area (right panel) and Cytokeratin-18 (lower panel). Right panels represent the quantification data using imageJ software. Statistical analysis was performed using one way ANOVA followed by post hoc Tukey test. (* p≤0.001). (B) RT² Profiler PCR Array was used to measure the mRNA expression of CXCL12, CCR4, HIF1α and the data was represented in heat map. (C) Real time PCR data of major gemcitabine metabolizing genes,β-actin was served as housekeeping gene, Statistical significance was calculated using t-test, * p≤0.001 vs vehicle. (D) Real time PCR data of showing the expression of Shh, Gli-1 and Gli-3 in pancreatic cancer tissue samples. β-actin was served as housekeeping gene, Statistical significance was calculated using t-test, * p≤0.001 vs vehicle.