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. 2018 Jul 1;8(14):4003–4015. doi: 10.7150/thno.24106

Figure 4.

Figure 4

BCL11A is a direct target gene of miR-4753 and miR-6809 and is inhibited by circEPSTI1 knockdown. (A) Venn diagram representing the overlap of co-target genes of miR-4753 and miR-6809 based on three algorithms (TargetScan (TS), MIRDB and TARGETMINER(TM)). The Venn diagram tool is available at http://bioinformatics.psb.ugent.be/webtools/Venn/. (B) Schematic of the predicted miR-4753 and miR-6809 sites in the 3′UTR of BCL11A mRNA. (C-D) Luciferase assay of HEK 293T cells co-transfected with a scrambled control, miR-4753 mimics, miR-6809 mimics, miR-4753+ miR-6809 mimics, control-LNA, miR-4753-LNA, or miR-4753+ miR-6809-LNA and a luciferase reporter containing BCL11A-3'UTR-wt or mutant constructs with mutated miR-4753 and miR-6809 binding sites (BCL11A-3'UTR-mut). (E) qRT-PCR analysis of BCL11A mRNA after transfection with scrambled control, miR-4753 mimics, miR-6809 mimics, miR-4753+ miR-6809 mimics, control-LNA, miR-4753-LNA or miR-4753+ miR-6809-LNA. (F-G) The effect of miR-4753 /miR-6809 mimics or miR-4753 /miR-6809 inhibitors on BCL11A protein expression in MDA-MB-231 cells was determined by Western blot. (H) The effect of knockdown of circEPSTI1 on BCL11A protein expression in MDA-MB-231 cells.