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Circulating Ects and plasma supernatant: workflow for miRNA extraction and analysis. Platelet-poor plasma (PPP) was harvested from blood samples of patients by centrifugation. The plasma was aliquoted in Eppendorf tubes (350 mL) and was stored at -80 °C. Ects were separated by centrifugation, and two plasma fractions, namely, Ect-enriched and Ect-depleted plasma fractions, were used for further analyses (adapted from 30).
