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. 2018 Jun 27;8(8):2833–2840. doi: 10.1534/g3.118.300448

Figure 2.

Figure 2

Off target detection in the PAX4 knockout rabbits. (A) T7E1 cleavage analysis of ten potential off-target sites (POTS) for sgRNA1 and sgRNA2. 1-5 represents the five POTS for sgRNA1, 6-10 represent the five POTS for sgRNA2. (B) Chromatogram sequence analysis of five POTS for sgRNA1 and sgRNA2 using PCR products in founders. The sequences of the POTS and the PAM are represented in shadow.