Table 1. Summary of the PAX4 KO rabbits generated by CRISPR/Cas9.
| Recipients | gRNA/Cas9 mRNA (ng/μl) | Embryos transferred | Pregnancy | Pups obtained (% transferred) | Pups with mutations (% pups) | Bi-allelic modified (% pups) | Pups with hyperglycemia (% pups) |
|---|---|---|---|---|---|---|---|
| 1 | 40/200 | 56 | YES | 8 (14.3%) | 8 (100%) | 8 (100%) | 8 (100%) |
| 2 | 40/200 | 52 | YES | 6 (11.5%) | 6 (100%) | 6 (100%) | 6 (100%) |
| 3 | 20/200 | 52 | YES | 7 (13.5%) | 4 (57.1%) | 1 (25%) | 1 (25%) |
| 4 | 20/200 | 50 | YES | 1 (2%) | 1 (100%) | 0 | 0 |
| Total | 210 | 100% | 22 (10.5%) | 19 (86.4%) | 15 (68.2%) | 15 (68.2%) |