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. 2018 Jun 8;8(8):2603–2615. doi: 10.1534/g3.118.200046

Table 1. sgRNA parameters used in this study.

Name Type1 Protospacer + PAM Specificity2 Chari3 Doench4 Mor.-Mateos5 Observed efficiency6 Efficiency median7 Chimerism8
VQ33-42 trugRNA G-N18 GATGAGGAGATATTATCTG AGG 95 79 72 57 75,0 92,2 5,4
VQ33-38 starts with G G-N19 GCCTTAACGTATTGATCATT AGG 96 2 36 28 84,4 94,6 5,9
VQ33-1 starts with G G-N19 GGGTCATCGTTGCTTCTCAG TGG 100 58 66 56 75,4 94,2 4,5
VQ19-6 starts with G G-N19 GGGACTGTTAAGTGCAAGCT TGG 99 28 48 45 34,4 19,6 3,5
VQ19-34 starts with G G-N19 GCGGAGAGTCTGGAGATCTT GGG 99 60 44 50 66,1 82,3 7,6
GRXS17-4 starts with G G-N19 GACCTTCGAGCCGAGCTCGG AGG 100 99 64 58 67,3 83,2 4,2
GLB3-3 starts with G G-N19 GATAAGGCATCGGTGTTAAG CGG 100 88 62 56 77,7 96,1 6,6
JAM2-109 starts with G G-N19 GGAGATTTGGTTCTCTGTTG GGG 97 31 48 53 88,6 97,7 3,4
JAM2-140 extra G G-N20 TATTGCAGAGAGCCTAAAGA AGG 96 80 56 36 26,4 4,5 2,5
GRXS17-133 extra G G-N20 CTTGATAACTTGCGCCAGAG CGG 84 86 62 57 NA NA NA
GRXS17-67 extra G G-N20 ATTATGGAGCTAAGTGAGAG TGG 98 87 63 28 NA NA NA
WRKY20-201 extra G G-N20 ACTTCCCAAAATGACTCCAG AGG 100 97 69 64 NA NA NA
WRKY20-39 starts with G G-N19 GTATGGCTGCACAAGAAGAA AGG 96 90 54 42 NA NA NA
1

, type of sgRNA depending on the position of the starting guanine nucleotide. 2, CRISPOR specificity score (0-100). 3, predicted efficiency score (0-100) by Chari et al. (2015). 4, predicted efficiency score (0-100) by Doench et al. (2014). 5, predicted efficiency score (0-100) by Moreno-Mateos et al. (2015). 6, observed efficiency (percentage of cells not WT) as the average efficiency indicated by TIDE T1 seedlings. 7, median efficiency indicated by TIDE T1 seedlings. 8, chimerism indicated as the average number of alleles present ≥ 1% in a T1 plant. NA, not applicable.