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Characterization of the cnp3-1 mutant. (A) The strains expressing GFP-tagged Cnp1 and mCherry-tagged Cnp3 (WT, MS548), Cnp3-1 mutant protein (cnp3-1, MS549) from the native promoter were grown at 26°C or 36°C for 24 hr and observed under a fluorescence microscope. The white arrow heads indicate mis-segregated chromatin mass scored as abnormal in (B). The bar indicates 10μm. (B) The statistic analysis of (A).
