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S508 of Cnp3 is important for centromere-targeting. Localization of a partial fragment of Cnp3 spanning from 414 to 643 amino acids tagged with GFP were examined at 26°C or 36°C in the wild type strain (MS568 and MS569). Sad1 tagged with mCherry was used as a marker for the centromere cluster. These strain were grown as in Fig. 1C. The bar indicates 5 μm.
