Table 1. S. pombe strains used in this study.
Strain | Genotype | Source and reference | |
---|---|---|---|
SP6 | h- | leu1-32 | Laboratory stock |
MS01 | h- | leu1-32 transformed with pREP41-GFP-H3.3 | This work |
MS02 | h- | leu1-32 transformed with pREP41-GFP-cnp1 | This work |
MS03 | h- | leu1-32 cnp3-1 transformed with pREP41-H3.3 | This work |
MS04 | h- | leu1-32 cnp3-1 transformed with pREP41-cnp3-1 | This work |
MS168 | h+ | leu1-32::nmtGFP-cnp1-leu1+ cnp3::kanR ade6-M216 | This work |
MS213 | h- | leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-mCherry-leu1+ | This work |
MS216 | h+ | leu1-32::nmtGFP-cnp1-leu1+ ade6-M216 | This work |
MS217 | h- | leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-1-mCherry-leu1+ | This work |
MS262 | h+ | leu1-32::nmtGFP-cnp1-leu1+ cnp3-1 ade6-M216 | This work |
MS343 | h- | leu1-32 GFP-cnp1-hph | Laboratory stock |
MS344 | h- | leu1-32 GFP-cnp1-hph cnp3-1 | This work |
MS413 | h- | leu1-32 cnp3-1 ade6-M210 ch16 | This work |
MS468 | h- | leu1-32 cnp3-GFP-leu1+ | This work |
MS497 | h- | leu1-32 GFP-cnp1-hph mis16-53 | This work |
MS503 | h- | leu1-32 GFP-cnp1-hph mis18-818 | This work |
MS508 | h | leu1-32 GFP-cnp1-hph mis6-302 | This work |
MS525 | h- | leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-3HA 6his-leu1+ | This work |
MS527 | h- | leu1-32 lys::nmtGFP-cnp1-lys1+ cnp3-1-3HA 6his-leu1+ | This work |
MS532 | h- | leu1-32 GFP-cnp1-hph mis6-302 cnp3-1 | This work |
MS533 | h- | leu1-32 GFP-cnp1-hph mis16-53 cnp3-1 | This wor |
MS534 | h- | leu1-32 GFP-cnp1-hph cnp3-1 mis18-818 | This work |
MS544 | h- | leu1-32 cnp3-1-GFP-leu1+ | This work |
MS545 | h- | leu1-32 GFP-cnp1-hph cnp3-1mCherry-leu1+ mis18-818 | This work |
MS546 | h- | leu1-32 GFP-cnp1-hph cnp3-mCherry-leu1+ mis18-818 | This work |
MS548 | h- | leu1-32 GFP-cnp1-hph cnp3-mCherry-leu1+ | This work |
MS549 | h- | leu1-32 GFP-cnp1-hph cnp3-1-mCherry-leu1+ | This work |
MS568 | h- | leu1-32 Sad1-mCherry<<kanR transformed with pREP81-cnp3-GFP | This work |
MS569 | h- | leu1-32 Sad1-mCherry<<kanR transformed with pREP81-cnp3-1-GFP(S508F) | This work |
MS615 | h- | leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ | This work |
MS616 | h- | leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ cnp3−1 | This work |
MS617 | h+ | leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+cnp3−1 mis18-818 | This work |
MS618 * | leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ mis18-818 | This work | |
MS620 | h- | leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ mis18-262 | This work |
MS621 | leu1-32 GFP-cnp1-hph mis12-mCherry-leu1+ cnp3−1mis18-262 | This work | |
TK5 | h- | leu1-32::nmt1GFP-cnp1-leu1+ | This work |
TK85 | h- | leu1-32 ade6-M210 ch16 | Laboratory stock |
: The mating type was not determined.