Table 3. Plasmids and primers used in this study.
*1 | pREP1-GFP-Cnp1 |
*2 | pREP1-GFP-Cnp1-pUM1 |
MS-1 | nmt1-GFP-cnp1-pBR322 |
MS-2 | cnp3-mCherry-pBS |
MS-3 | cnp3-1mCherry-pBS |
MS-4 | cnp3-HA-pBS |
MS-5 | cnp3-1HA-pBS |
MS-6 | pREP81-cnp3-GFP |
MS-7 | pREP81-cnp3-1-GFP |
Cnp1-F | ATAGTCGACTATGGCAAAGAAATCTTTAATG |
Cnp1-R | ATAGCGGCCGCTCAAGCACCACGAATCC |
GFP-Cnp1-F | ATAGGATCCTATGAGTAAAGGAGAAGAAC |
GFP-Cnp1-R | ATAGGATCCTCAAGCACCACGAATCCTC |
Cnp3-F | ATACTCGAGATGTAGACGATGAATGAAACGTC |
Cnp3-R | ATAGCGGCCGCGTCGTTCGTTTGGAAAATC |
Rep81Cnp3-F | ATACATATGTTCTAGAGATGAAGATTCCG |
Rep81-Cnp3-R | ATAGCGGCCGCGTCGYYCGTTTGGAAAATC |
1, 2 (Kitagawa et al. 2014)